Uloga polimorfizama gena adrb3, actn3 i ace u pokazateljima mišićnoga umora nakon ekscentrično-koncentričnih kontrakcija

doctoral thesis

• Title (english): The role of adrb3, actn3 and ace genetic polymorphisms in muscle fatigue after eccentric-concentric contractions
• Author: Vladimir Manojlović
• Mentor: Lana Ružić (mentor)
• Mentor: Pavle Mikulić (komentor)
• Committee member: Branka Matković (predsjednik povjerenstva)
• Committee member: Ljubomir Antekolović (član povjerenstva)
• Committee member: Branko Malojčić (član povjerenstva); MBZ: 253095
• Granter: University of Zagreb; Faculty of Kinesiology; Zagreb
• Defense date and country: 2016-06-28, Croatia
• Scientific / art field, discipline and subdiscipline: SOCIAL SCIENCES; Kinesiology
• Universal decimal classification (UDC): 612 - Physiology ; 796/799 - Sport

Abstract

Cilj rada je bio utvrditi ulogu polimorfizama ADRB3, ACTN3 i ACE gena u pokazateljima mišićnoga umora i oštećenja nakon pliometrijskog trenažnog protokola do otkaza. Pokazatelji oštećenja bili su kreatin kinaza (CK), aspartat aminotransferaza (AST), mioglobin (Mb), C-reaktivni protein (CRP) i VAS skala (skala subjektivnog doživljaja boli).Uzorak ispitanika se sastojao od 43 tjelesno aktivne osobe muškog spola [dob: 20,0 (19,0 22,0) god., tjelesna masa: 81,7 8,0 kg i visina: 184,1 7,5 cm]. Trenažni protokol se sastojao od tri serije maksimalnih sunožnih skokova s pripremom bez zamaha ruku do otkaza. Prije početka protokola uzet je uzorak brisa bukalne sluznice za DNK analizu te su izmjerene razine CK, AST, Mb i CRP. Mjerenje parametara u krvi ponovljeno je 48 sati nakon protokola. Odmah nakon protokola izmjerena je koncentracija laktata u krvi, a nakon 48 sati očitane su vrijednosti na skali za subjektivnu procjenu mišićne boli. Hipoteze su pretpostavljale da će genotipovi jakosti/brzine ADRB3, ACTN3 i ACE gena, kao i ACE + ACTN3 kombinacija jakosti i brzine, imati manji porast vrijednosti mišićnog oštećenja od genotipova izdržljivosti i ACE + ACTN3 kombinacije izdržljivosti.ADRB3 Trp/Arg genotip imao je značajno više finalne vrijednosti Mb (p = 0,04), laktata u krvi (p = 0,01) i subjektivnog doživljaja mišićne boli (p = 0,05), kao i tendenciju prema značajno višim vrijednostima CK u usporedbi s Trp/Trp genotipom (p = 0,07). Značajno više vrijednosti AST u inicijalnom mjerenju imao je ACE ID genotip u usporedbi s II (p = 0,02) i DD (p = 0,03) genotipovima. ACE DD genotip imao je značajno više vrijednosti od II genotipa u inicijalnom mjerenju CRP (p = 0,04) te tendenciju prema značajno višim vrijednostima od ID genotipa (p = 0,06).Kombinacija genotipova jakosti/brzine je pokazala tendenciju prema značajno višim vrijednostima CRP u inicijalnom mjerenju u usporedbi s kombinacijom jakosti/brzine i izdržljivosti (p = 0,06).
Na temelju dobivenih značajnih razlika zaključuje se kako je Trp/Arg genotip pokazao manju toleranciju na trenažni protokol pliometrijskog karaktera od Trp/Trp genotipa. S obzirom na nedostatan broj istraživanja ADRB3 Trp64Arg polimorfizma u sportu, dobiveni rezultati mogu poslužiti kao podloga za buduće radove na ovu temu.

Abstract (english)

The aim of the work was to determine the role of ADRB3, ACTN3 and ACE genetic polymorphisms in muscle damage markers following a plyometric traning protocol to exhaustion. The damage markers used were creatine kinase (CK), aspartate aminotransferase (AST), myoglobin (Mb), C-reactive protein (CRP) and the perception of muscle soreness as evaluated using the VAS scale.Forty three physically active university students participated in the study [age: 20,0 (19,0 22,0) years, body mass: 81,7 8,0 kg and height: 184,1 7,5 cm]. Training protocol consisted of three series of maximal bilateral countermovement jump (CMJ) without arm swing to exhaustion. CK, AST, Mb and CRP blood sample, as well as DNA sample were taken before the protocol. The blood sample was taken again 48h after the protocol. Blood lactate sample was taken after the protocol and VAS values were taken 48h after the protocol. We hypothesized that power/hypertrophy genotypes of ADRB3, ACTN3 and ACE genes, as well as ACE + ACTN3 power/hypertrophycombination, would have smaller changes of muscular damage compared with endurance genotypes and ACE + ACTN3 endurance combination. ADRB3 Trp/Arg genotype had significantly higher final Mb (p = 0,04), lactate (p = 0,01), and muscle pain (p = 0,05) values, as well as a tendency toward significantly higher CK values compared with Trp/Trp genotype (p = 0,07). ACE ID genotype had significantly higher initial AST values compared with II (p = 0,02) and DD (p = 0,03) genotypes. ACE DD genotype had significantly higher initial CRP values compared with II genotype (p = 0,04) and a tendency toward significantly higher values compared with ID genotype (p = 0,06). Power and hypertrophy combination showed a tendency toward significantly higher initial CRP values compared with endurance/power combination (p = 0,06). In conclusion, the ADRB3 Trp/Arg genotype showed lower tolerance to the plyometric training protocol in comparison with the Trp/Trp genotype. Given the scarcity of research concerning the ADRB3 Trp64Arg polymorphism in sport, this article can serve as the base for further work in this area.

• Keywords: polimorfizam; mišićni umor; pliometrijski skokovi; biokemijski pokazatelji
• Keywords (english): polymorphism; muscular fatigue; plyometric jumps; muscle damage markers
• Language: croatian
• URN:NBN: urn:nbn:hr:117:593484
• Study programme: Title: postgraduate doctoral study of kinesiology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje društvenih znanosti, polje kineziologija (doktor/doktorica znanosti, područje društvenih znanosti, polje kineziologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-01-12 14:04:02